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Image Search Results


Journal: eLife

Article Title: Cytoneme delivery of Sonic Hedgehog from ligand-producing cells requires Myosin 10 and a Dispatched-BOC/CDON co-receptor complex

doi: 10.7554/eLife.61432

Figure Lengend Snippet:

Article Snippet: The following plasmids were used in this study: pCDNA (control vector) (Clontech), pCDNA3-EGFP (Addgene Plasmid #13031), pCMV-mCherry-Mem (Addgene Plasmid #55779), pcDNA-Wnt3A (Addgene Plasmid #35908), pIRES-Jag1-HA (Addgene Plasmid #17336), pCMV-R-GECO1 (Addgene Plasmid #32444), pCMV-mCherry-CD9 (Addgene Plasmid #55013), pCMV-mCherry-CD81 (Addgene Plasmid #55012), pEGFP-CD63-C2 (Addgene Plasmid #62964), pCMV-EGFP-Rab18 (Addgene Plasmid #49550), pEGFP-C1-hMyoX (Addgene Plasmid #47608), pCMV6-hGAS1-Myc-DDK (Origene Cat: RC224804), pCMV3-mFGF2-N-GFPSpark (SinoBiological Cat: MG50037-ANG), pCMV3-mBMP2-C-GFPSpark (SinoBiological Cat: MG51115-ACG), pCMV-mCherry2, pCDNA3-mSHH-FL, pCDNA3-mSHH-N, pCDNA3-mSHH-FL-EGFP, pCDNA3-mSHH-FL-mCherry2, pCDNA3-V5-Disp-HA, pCS2-hBOC-EGFP and pCS2-hCDON-EGFP (a gift from A. Salic), pEGFP-C2-bMyo10-HMM ( ) and pEGFP-C2-bMyo10-Δ3PH, a modified version of pEGFP-bMyo10 where the 3 PH domains were removed via deletion of aa 1168–1491.

Techniques: Derivative Assay, Transfection, Construct, Recombinant, In Situ, Software, Imaging, Luciferase, Reporter Assay, Western Blot

Journal: eLife

Article Title: Cytoneme delivery of Sonic Hedgehog from ligand-producing cells requires Myosin 10 and a Dispatched-BOC/CDON co-receptor complex

doi: 10.7554/eLife.61432

Figure Lengend Snippet:

Article Snippet: The following plasmids were used in this study: pCDNA (control vector) (Clontech), pCDNA3-EGFP (Addgene Plasmid #13031), pCMV-mCherry-Mem (Addgene Plasmid #55779), pcDNA-Wnt3A (Addgene Plasmid #35908), pIRES-Jag1-HA (Addgene Plasmid #17336), pCMV-R-GECO1 (Addgene Plasmid #32444), pCMV-mCherry-CD9 (Addgene Plasmid #55013), pCMV-mCherry-CD81 (Addgene Plasmid #55012), pEGFP-CD63-C2 (Addgene Plasmid #62964), pCMV-EGFP-Rab18 (Addgene Plasmid #49550), pEGFP-C1-hMyoX (Addgene Plasmid #47608), pCMV6-hGAS1-Myc-DDK (Origene Cat: RC224804), pCMV3-mFGF2-N-GFPSpark (SinoBiological Cat: MG50037-ANG), pCMV3-mBMP2-C-GFPSpark (SinoBiological Cat: MG51115-ACG), pCMV-mCherry2, pCDNA3-mSHH-FL, pCDNA3-mSHH-N, pCDNA3-mSHH-FL-EGFP, pCDNA3-mSHH-FL-mCherry2, pCDNA3-V5-Disp-HA, pCS2-hBOC-EGFP and pCS2-hCDON-EGFP (a gift from A. Salic), pEGFP-C2-bMyo10-HMM ( ) and pEGFP-C2-bMyo10-Δ3PH, a modified version of pEGFP-bMyo10 where the 3 PH domains were removed via deletion of aa 1168–1491.

Techniques: Derivative Assay, Transfection, Construct, Recombinant, In Situ, Software, Imaging, Luciferase, Reporter Assay, Western Blot

Journal: eLife

Article Title: Cytoneme delivery of Sonic Hedgehog from ligand-producing cells requires Myosin 10 and a Dispatched-BOC/CDON co-receptor complex

doi: 10.7554/eLife.61432

Figure Lengend Snippet:

Article Snippet: The following plasmids were used in this study: pCDNA (control vector) (Clontech), pCDNA3-EGFP (Addgene Plasmid #13031), pCMV-mCherry-Mem (Addgene Plasmid #55779), pcDNA-Wnt3A (Addgene Plasmid #35908), pIRES-Jag1-HA (Addgene Plasmid #17336), pCMV-R-GECO1 (Addgene Plasmid #32444), pCMV-mCherry-CD9 (Addgene Plasmid #55013), pCMV-mCherry-CD81 (Addgene Plasmid #55012), pEGFP-CD63-C2 (Addgene Plasmid #62964), pCMV-EGFP-Rab18 (Addgene Plasmid #49550), pEGFP-C1-hMyoX (Addgene Plasmid #47608), pCMV6-hGAS1-Myc-DDK (Origene Cat: RC224804), pCMV3-mFGF2-N-GFPSpark (SinoBiological Cat: MG50037-ANG), pCMV3-mBMP2-C-GFPSpark (SinoBiological Cat: MG51115-ACG), pCMV-mCherry2, pCDNA3-mSHH-FL, pCDNA3-mSHH-N, pCDNA3-mSHH-FL-EGFP, pCDNA3-mSHH-FL-mCherry2, pCDNA3-V5-Disp-HA, pCS2-hBOC-EGFP and pCS2-hCDON-EGFP (a gift from A. Salic), pEGFP-C2-bMyo10-HMM ( ) and pEGFP-C2-bMyo10-Δ3PH, a modified version of pEGFP-bMyo10 where the 3 PH domains were removed via deletion of aa 1168–1491.

Techniques: Derivative Assay, Transfection, Construct, Recombinant, In Situ, Software, Imaging, Luciferase, Reporter Assay, Western Blot

Journal: eLife

Article Title: Cytoneme delivery of Sonic Hedgehog from ligand-producing cells requires Myosin 10 and a Dispatched-BOC/CDON co-receptor complex

doi: 10.7554/eLife.61432

Figure Lengend Snippet:

Article Snippet: The following plasmids were used in this study: pCDNA (control vector) (Clontech), pCDNA3-EGFP (Addgene Plasmid #13031), pCMV-mCherry-Mem (Addgene Plasmid #55779), pcDNA-Wnt3A (Addgene Plasmid #35908), pIRES-Jag1-HA (Addgene Plasmid #17336), pCMV-R-GECO1 (Addgene Plasmid #32444), pCMV-mCherry-CD9 (Addgene Plasmid #55013), pCMV-mCherry-CD81 (Addgene Plasmid #55012), pEGFP-CD63-C2 (Addgene Plasmid #62964), pCMV-EGFP-Rab18 (Addgene Plasmid #49550), pEGFP-C1-hMyoX (Addgene Plasmid #47608), pCMV6-hGAS1-Myc-DDK (Origene Cat: RC224804), pCMV3-mFGF2-N-GFPSpark (SinoBiological Cat: MG50037-ANG), pCMV3-mBMP2-C-GFPSpark (SinoBiological Cat: MG51115-ACG), pCMV-mCherry2, pCDNA3-mSHH-FL, pCDNA3-mSHH-N, pCDNA3-mSHH-FL-EGFP, pCDNA3-mSHH-FL-mCherry2, pCDNA3-V5-Disp-HA, pCS2-hBOC-EGFP and pCS2-hCDON-EGFP (a gift from A. Salic), pEGFP-C2-bMyo10-HMM ( ) and pEGFP-C2-bMyo10-Δ3PH, a modified version of pEGFP-bMyo10 where the 3 PH domains were removed via deletion of aa 1168–1491.

Techniques: Derivative Assay, Transfection, Construct, Recombinant, In Situ, Software, Imaging, Luciferase, Reporter Assay, Western Blot

Journal: eLife

Article Title: Cytoneme delivery of Sonic Hedgehog from ligand-producing cells requires Myosin 10 and a Dispatched-BOC/CDON co-receptor complex

doi: 10.7554/eLife.61432

Figure Lengend Snippet:

Article Snippet: The following plasmids were used in this study: pCDNA (control vector) (Clontech), pCDNA3-EGFP (Addgene Plasmid #13031), pCMV-mCherry-Mem (Addgene Plasmid #55779), pcDNA-Wnt3A (Addgene Plasmid #35908), pIRES-Jag1-HA (Addgene Plasmid #17336), pCMV-R-GECO1 (Addgene Plasmid #32444), pCMV-mCherry-CD9 (Addgene Plasmid #55013), pCMV-mCherry-CD81 (Addgene Plasmid #55012), pEGFP-CD63-C2 (Addgene Plasmid #62964), pCMV-EGFP-Rab18 (Addgene Plasmid #49550), pEGFP-C1-hMyoX (Addgene Plasmid #47608), pCMV6-hGAS1-Myc-DDK (Origene Cat: RC224804), pCMV3-mFGF2-N-GFPSpark (SinoBiological Cat: MG50037-ANG), pCMV3-mBMP2-C-GFPSpark (SinoBiological Cat: MG51115-ACG), pCMV-mCherry2, pCDNA3-mSHH-FL, pCDNA3-mSHH-N, pCDNA3-mSHH-FL-EGFP, pCDNA3-mSHH-FL-mCherry2, pCDNA3-V5-Disp-HA, pCS2-hBOC-EGFP and pCS2-hCDON-EGFP (a gift from A. Salic), pEGFP-C2-bMyo10-HMM ( ) and pEGFP-C2-bMyo10-Δ3PH, a modified version of pEGFP-bMyo10 where the 3 PH domains were removed via deletion of aa 1168–1491.

Techniques: Derivative Assay, Transfection, Construct, Recombinant, In Situ, Software, Imaging, Luciferase, Reporter Assay, Western Blot

Journal: eLife

Article Title: Cytoneme delivery of Sonic Hedgehog from ligand-producing cells requires Myosin 10 and a Dispatched-BOC/CDON co-receptor complex

doi: 10.7554/eLife.61432

Figure Lengend Snippet:

Article Snippet: The following plasmids were used in this study: pCDNA (control vector) (Clontech), pCDNA3-EGFP (Addgene Plasmid #13031), pCMV-mCherry-Mem (Addgene Plasmid #55779), pcDNA-Wnt3A (Addgene Plasmid #35908), pIRES-Jag1-HA (Addgene Plasmid #17336), pCMV-R-GECO1 (Addgene Plasmid #32444), pCMV-mCherry-CD9 (Addgene Plasmid #55013), pCMV-mCherry-CD81 (Addgene Plasmid #55012), pEGFP-CD63-C2 (Addgene Plasmid #62964), pCMV-EGFP-Rab18 (Addgene Plasmid #49550), pEGFP-C1-hMyoX (Addgene Plasmid #47608), pCMV6-hGAS1-Myc-DDK (Origene Cat: RC224804), pCMV3-mFGF2-N-GFPSpark (SinoBiological Cat: MG50037-ANG), pCMV3-mBMP2-C-GFPSpark (SinoBiological Cat: MG51115-ACG), pCMV-mCherry2, pCDNA3-mSHH-FL, pCDNA3-mSHH-N, pCDNA3-mSHH-FL-EGFP, pCDNA3-mSHH-FL-mCherry2, pCDNA3-V5-Disp-HA, pCS2-hBOC-EGFP and pCS2-hCDON-EGFP (a gift from A. Salic), pEGFP-C2-bMyo10-HMM ( ) and pEGFP-C2-bMyo10-Δ3PH, a modified version of pEGFP-bMyo10 where the 3 PH domains were removed via deletion of aa 1168–1491.

Techniques: Derivative Assay, Transfection, Construct, Recombinant, In Situ, Software, Imaging, Luciferase, Reporter Assay, Western Blot

Journal: eLife

Article Title: Cytoneme delivery of Sonic Hedgehog from ligand-producing cells requires Myosin 10 and a Dispatched-BOC/CDON co-receptor complex

doi: 10.7554/eLife.61432

Figure Lengend Snippet:

Article Snippet: The following plasmids were used in this study: pCDNA (control vector) (Clontech), pCDNA3-EGFP (Addgene Plasmid #13031), pCMV-mCherry-Mem (Addgene Plasmid #55779), pcDNA-Wnt3A (Addgene Plasmid #35908), pIRES-Jag1-HA (Addgene Plasmid #17336), pCMV-R-GECO1 (Addgene Plasmid #32444), pCMV-mCherry-CD9 (Addgene Plasmid #55013), pCMV-mCherry-CD81 (Addgene Plasmid #55012), pEGFP-CD63-C2 (Addgene Plasmid #62964), pCMV-EGFP-Rab18 (Addgene Plasmid #49550), pEGFP-C1-hMyoX (Addgene Plasmid #47608), pCMV6-hGAS1-Myc-DDK (Origene Cat: RC224804), pCMV3-mFGF2-N-GFPSpark (SinoBiological Cat: MG50037-ANG), pCMV3-mBMP2-C-GFPSpark (SinoBiological Cat: MG51115-ACG), pCMV-mCherry2, pCDNA3-mSHH-FL, pCDNA3-mSHH-N, pCDNA3-mSHH-FL-EGFP, pCDNA3-mSHH-FL-mCherry2, pCDNA3-V5-Disp-HA, pCS2-hBOC-EGFP and pCS2-hCDON-EGFP (a gift from A. Salic), pEGFP-C2-bMyo10-HMM ( ) and pEGFP-C2-bMyo10-Δ3PH, a modified version of pEGFP-bMyo10 where the 3 PH domains were removed via deletion of aa 1168–1491.

Techniques: Derivative Assay, Transfection, Construct, Recombinant, In Situ, Software, Imaging, Luciferase, Reporter Assay, Western Blot

Journal: eLife

Article Title: Cytoneme delivery of Sonic Hedgehog from ligand-producing cells requires Myosin 10 and a Dispatched-BOC/CDON co-receptor complex

doi: 10.7554/eLife.61432

Figure Lengend Snippet:

Article Snippet: The following plasmids were used in this study: pCDNA (control vector) (Clontech), pCDNA3-EGFP (Addgene Plasmid #13031), pCMV-mCherry-Mem (Addgene Plasmid #55779), pcDNA-Wnt3A (Addgene Plasmid #35908), pIRES-Jag1-HA (Addgene Plasmid #17336), pCMV-R-GECO1 (Addgene Plasmid #32444), pCMV-mCherry-CD9 (Addgene Plasmid #55013), pCMV-mCherry-CD81 (Addgene Plasmid #55012), pEGFP-CD63-C2 (Addgene Plasmid #62964), pCMV-EGFP-Rab18 (Addgene Plasmid #49550), pEGFP-C1-hMyoX (Addgene Plasmid #47608), pCMV6-hGAS1-Myc-DDK (Origene Cat: RC224804), pCMV3-mFGF2-N-GFPSpark (SinoBiological Cat: MG50037-ANG), pCMV3-mBMP2-C-GFPSpark (SinoBiological Cat: MG51115-ACG), pCMV-mCherry2, pCDNA3-mSHH-FL, pCDNA3-mSHH-N, pCDNA3-mSHH-FL-EGFP, pCDNA3-mSHH-FL-mCherry2, pCDNA3-V5-Disp-HA, pCS2-hBOC-EGFP and pCS2-hCDON-EGFP (a gift from A. Salic), pEGFP-C2-bMyo10-HMM ( ) and pEGFP-C2-bMyo10-Δ3PH, a modified version of pEGFP-bMyo10 where the 3 PH domains were removed via deletion of aa 1168–1491.

Techniques: Derivative Assay, Transfection, Construct, Recombinant, In Situ, Software, Imaging, Luciferase, Reporter Assay, Western Blot